{"id":1587,"date":"2021-05-12T15:20:36","date_gmt":"2021-05-12T15:20:36","guid":{"rendered":"https:\/\/translate.peaches.es\/?p=1587"},"modified":"2022-11-21T10:59:18","modified_gmt":"2022-11-21T10:59:18","slug":"pdx-derived-organoids-model-in-vivo-drug-response-and-secrete-biomarkers","status":"publish","type":"post","link":"https:\/\/peaches.es\/en\/pdx-derived-organoids-model-in-vivo-drug-response-and-secrete-biomarkers\/","title":{"rendered":"PDX-derived organoids model in vivo drug response and secrete biomarkers"},"content":{"rendered":"<header id=\"heading\" class=\"heading\">\n<div id=\"full-view-heading\" class=\"full-view\">\n<div class=\"article-citation\">\n<div>Publicado por <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/\" target=\"_blank\" rel=\"noopener noreferrer\">PubMed<\/a><\/div>\n<div><\/div>\n<div class=\"article-source\"><span class=\"cit\">2020 Nov 5;5(21):e135544.<\/span><\/div>\n<p><span class=\"citation-doi\">doi: 10.1172\/jci.insight.135544.<\/span><\/p>\n<\/div>\n<h3 class=\"heading-title\">PDX-derived organoids model in vivo drug response and secrete biomarkers<\/h3>\n<div class=\"inline-authors\">\n<div class=\"authors\">\n<div class=\"authors-list\"><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Huang+L&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Ling Huang\">Ling Huang<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Bockorny+B&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Bruno Bockorny\">Bruno Bockorny<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Paul+I&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Indranil Paul\">Indranil Paul<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Departments of Biology and Biochemistry, Boston University, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-3\">3<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Akshinthala+D&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Dipikaa Akshinthala\">Dipikaa Akshinthala<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Frappart+PO&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Pierre-Oliver Frappart\">Pierre-Oliver Frappart<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Internal Medicine I, University Hospital Ulm, Ulm, Germany.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-4\">4<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Gandarilla+O&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Omar Gandarilla\">Omar Gandarilla<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Bose+A&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Arindam Bose\">Arindam Bose<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Sanchez-Gonzalez+V&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Veronica Sanchez-Gonzalez\">Veronica Sanchez-Gonzalez<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"NanoView Biosciences, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-5\">5<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Rouse+EE&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Emily E Rouse\">Emily E Rouse<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Surgery and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-6\">6<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Lehoux+SD&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Sylvain D Lehoux\">Sylvain D Lehoux<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Surgery and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-6\">6<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Pandell+N&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Nicole Pandell\">Nicole Pandell<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Lim+CM&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Christine M Lim\">Christine M Lim<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Clohessy+JG&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"John G Clohessy\">John G Clohessy<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Grossman+J&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Joseph Grossman\">Joseph Grossman<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Gonzalez+R&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Raul Gonzalez\">Raul Gonzalez<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-7\">7<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Del+Pino+SP&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Sofia Perea Del Pino\">Sofia Perea Del Pino<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Daaboul+G&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"George Daaboul\">George Daaboul<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"NanoView Biosciences, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-5\">5<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Sawhney+MS&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Mandeep S Sawhney\">Mandeep S Sawhney<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Freedman+SD&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Steven D Freedman\">Steven D Freedman<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Kleger+A&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Alexander Kleger\">Alexander Kleger<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Internal Medicine I, University Hospital Ulm, Ulm, Germany.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-4\">4<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Cummings+RD&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Richard D Cummings\">Richard D Cummings<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Surgery and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-6\">6<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Emili+A&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Andrew Emili\">Andrew Emili<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Departments of Biology and Biochemistry, Boston University, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-3\">3<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Muthuswamy+LB&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Lakshmi B Muthuswamy\">Lakshmi B Muthuswamy<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Hidalgo+M&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Manuel Hidalgo\">Manuel Hidalgo<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><span class=\"comma\">,\u00a0<\/span><\/span><span class=\"authors-list-item \"><a class=\"full-name\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Muthuswamy+SK&amp;cauthor_id=32990680\" data-ga-category=\"search\" data-ga-action=\"author_link\" data-ga-label=\"Senthil K Muthuswamy\">Senthil K Muthuswamy<\/a><sup class=\"affiliation-links\"><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Cancer Center and.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-1\">1<\/a><span class=\"author-sup-separator\">\u00a0<\/span><a class=\"affiliation-link\" title=\"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32990680\/#affiliation-2\">2<\/a><\/sup><\/span><\/div>\n<\/div>\n<\/div>\n<div class=\"short-article-details\">Affiliations <button id=\"toggle-authors\" class=\"more-details\" data-alt-text=\"Collapse\" aria-controls=\"expanded-authors\" aria-expanded=\"false\" data-pinger-ignore=\"\">expand<\/button><\/div>\n<ul id=\"full-view-identifiers\" class=\"identifiers\">\n<li><span class=\"identifier pubmed\"><span class=\"id-label\">PMID: <\/span><strong class=\"current-id\" title=\"PubMed ID\">32990680<\/strong><\/span><\/li>\n<li><span class=\"identifier pmc\"><span class=\"id-label\">PMCID: <\/span><a class=\"id-link\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc7710298\/\" target=\"_blank\" rel=\"noopener noreferrer\" data-ga-category=\"full_text\" data-ga-action=\"PMCID\">PMC7710298<\/a><\/span><\/li>\n<li><span class=\"identifier doi\"><span class=\"id-label\">DOI: <\/span><a class=\"id-link\" href=\"https:\/\/doi.org\/10.1172\/jci.insight.135544\" target=\"_blank\" rel=\"noopener noreferrer\" data-ga-category=\"full_text\" data-ga-action=\"DOI\">10.1172\/jci.insight.135544<\/a><\/span><\/li>\n<\/ul>\n<p><span class=\"free-label\">Free PMC article<\/span><\/p>\n<\/div>\n<\/header>\n<div id=\"abstract\" class=\"abstract\">\n<h3 class=\"title\">Abstract<\/h3>\n<div id=\"enc-abstract\" class=\"abstract-content selected\">\n<p>Patient-derived organoid models are proving to be a powerful platform for both basic and translational studies. Here we conduct a methodical analysis of pancreatic ductal adenocarcinoma (PDAC) tumor organoid drug response in paired patient-derived xenograft (PDX) and PDX-derived organoid (PXO) models grown under WNT-free culture conditions. We report a specific relationship between area under the curve value of organoid drug dose response and in vivo tumor growth, irrespective of the drug treatment. In addition, we analyzed the glycome of PDX and PXO models and demonstrate that PXOs recapitulate the in vivo glycan landscape. In addition, we identify a core set of 57 N-glycans detected in all 10 models that represent 50%-94% of the relative abundance of all N-glycans detected in each of the models. Last, we developed a secreted biomarker discovery pipeline using media supernatant of organoid cultures and identified potentially new extracellular vesicle (EV) protein markers. We validated our findings using plasma samples from patients with PDAC, benign gastrointestinal diseases, and chronic pancreatitis and discovered that 4 EV proteins are potential circulating biomarkers for PDAC. Thus, we demonstrate the utility of organoid cultures to not only model in vivo drug responses but also serve as a powerful platform for discovering clinically actionable serologic biomarkers.<\/p>\n<\/div>\n<p><strong class=\"sub-title\">Keywords: <\/strong>Cancer; Glycobiology; Oncology.<\/p>\n<\/div>\n<div id=\"conflict-of-interest\" class=\"conflict-of-interest\">\n<h3 class=\"title\">Conflict of interest statement<\/h3>\n<div class=\"statement\">\n<p>Conflict of interest: LH and SKM have a patent application pending for the pancreas tumor organoid media used in this study (US20170267977A1). BB received research funding from NanoView Biosciences. MH has stock and ownership interests in Champions Oncology, PharmaCyte Biotech, Bioncotech, Nelum, and Agenus; his honoraria include Takeda, Agenus, InxMed, PharmaCyte Biotech, Bioncotech, Tolero, Novartis, Oncomatrix, and KAHR Medical; he has a consulting or advisory role for Takeda, Agenus, InxMed, PharmaCyte Biotech, Bioncotech, Tolero, Novartis, Oncomatrix, and KAHR Medical; his patents, royalties, and other intellectual property include Myriad Genetics.<\/p>\n<\/div>\n<\/div>\n<div id=\"figures\" class=\"figures\">\n<h3 class=\"title\">Figures<\/h3>\n<div id=\"slides-container\" class=\"figures-list\">\n<figure class=\"figure-item \" data-slide-index=\"0\" data-label-slug=\"figure-1\"><a class=\"figure-link\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g001.jpg\" aria-describedby=\"figure-caption-0\" data-image-width=\"753\" data-image-height=\"936\" data-image-alt=\"Figure 1\" data-pmc-id=\"PMC7710298\" data-figure-id=\"F1\"><img decoding=\"async\" id=\"article-image-0\" class=\"figure-thumb\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g001.gif\" alt=\"Figure 1\" \/><\/a><\/p>\n<div class=\"figure-caption figure-caption-text\" aria-hidden=\"true\">\n<div class=\"caption-wrap\">\n<p>Figure 1. Genomic and histological features of\u2026<\/p>\n<\/div>\n<\/div>\n<\/figure>\n<figure class=\"figure-item \" data-slide-index=\"1\" data-label-slug=\"figure-2\"><a class=\"figure-link\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g002.jpg\" aria-describedby=\"figure-caption-1\" data-image-width=\"747\" data-image-height=\"690\" data-image-alt=\"Figure 2\" data-pmc-id=\"PMC7710298\" data-figure-id=\"F2\"><img decoding=\"async\" id=\"article-image-1\" class=\"figure-thumb\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g002.gif\" alt=\"Figure 2\" \/><\/a><\/p>\n<div class=\"figure-caption figure-caption-text\" aria-hidden=\"true\">\n<div class=\"caption-wrap\">\n<p>Figure 2. Differences in biology of organoids\u2026<\/p>\n<\/div>\n<\/div>\n<\/figure>\n<figure class=\"figure-item \" data-slide-index=\"2\" data-label-slug=\"figure-3\"><a class=\"figure-link\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g003.jpg\" aria-describedby=\"figure-caption-2\" data-image-width=\"738\" data-image-height=\"972\" data-image-alt=\"Figure 3\" data-pmc-id=\"PMC7710298\" data-figure-id=\"F3\"><img decoding=\"async\" id=\"article-image-2\" class=\"figure-thumb\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g003.gif\" alt=\"Figure 3\" \/><\/a><\/p>\n<div class=\"figure-caption figure-caption-text\" aria-hidden=\"true\">\n<div class=\"caption-wrap\">\n<p>Figure 3. Concordance between PXO and PDX\u2026<\/p>\n<\/div>\n<\/div>\n<\/figure>\n<figure class=\"figure-item \" data-slide-index=\"3\" data-label-slug=\"figure-4\"><a class=\"figure-link\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g004.jpg\" aria-describedby=\"figure-caption-3\" data-image-width=\"758\" data-image-height=\"1908\" data-image-alt=\"Figure 4\" data-pmc-id=\"PMC7710298\" data-figure-id=\"F4\"><img decoding=\"async\" id=\"article-image-3\" class=\"figure-thumb\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g004.gif\" alt=\"Figure 4\" \/><\/a><\/p>\n<div class=\"figure-caption figure-caption-text\" aria-hidden=\"true\">\n<div class=\"caption-wrap\">\n<p>Figure 4. PXO AUC values can predict\u2026<\/p>\n<\/div>\n<\/div>\n<\/figure>\n<figure class=\"figure-item \" data-slide-index=\"4\" data-label-slug=\"figure-5\"><a class=\"figure-link\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g005.jpg\" aria-describedby=\"figure-caption-4\" data-image-width=\"740\" data-image-height=\"714\" data-image-alt=\"Figure 5\" data-pmc-id=\"PMC7710298\" data-figure-id=\"F5\"><img decoding=\"async\" id=\"article-image-4\" class=\"figure-thumb\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g005.gif\" alt=\"Figure 5\" \/><\/a><\/p>\n<div class=\"figure-caption figure-caption-text\" aria-hidden=\"true\">\n<div class=\"caption-wrap\">\n<p>Figure 5. N-glycan profiles of PXO and\u2026<\/p>\n<\/div>\n<\/div>\n<\/figure>\n<figure class=\"figure-item \" data-slide-index=\"5\" data-label-slug=\"figure-6\"><a class=\"figure-link\" role=\"button\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g006.jpg\" aria-describedby=\"figure-caption-5\" data-image-width=\"755\" data-image-height=\"596\" data-image-alt=\"Figure 6\" data-pmc-id=\"PMC7710298\" data-figure-id=\"F6\"><img decoding=\"async\" id=\"article-image-5\" class=\"figure-thumb\" src=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/instance\/7710298\/bin\/jciinsight-5-135544-g006.gif\" alt=\"Figure 6\" \/><\/a><\/p>\n<div class=\"figure-caption figure-caption-text\" aria-hidden=\"true\">\n<div class=\"caption-wrap\">\n<p>Figure 6. Comparison of N-glycan subtypes and\u2026<\/p>\n<\/div>\n<\/div>\n<\/figure>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Publicado por PubMed 2020 Nov 5;5(21):e135544. doi: 10.1172\/jci.insight.135544. PDX-derived organoids model in vivo drug response and secrete biomarkers Ling Huang\u00a01\u00a02,\u00a0Bruno Bockorny\u00a01\u00a02,\u00a0Indranil Paul\u00a03,\u00a0Dipikaa Akshinthala\u00a01\u00a02,\u00a0Pierre-Oliver Frappart\u00a04,\u00a0Omar Gandarilla\u00a01\u00a02,\u00a0Arindam Bose\u00a01\u00a02,\u00a0Veronica Sanchez-Gonzalez\u00a05,\u00a0Emily E Rouse\u00a06,\u00a0Sylvain D Lehoux\u00a06,\u00a0Nicole Pandell\u00a01\u00a02,\u00a0Christine M Lim\u00a01\u00a02,\u00a0John G Clohessy\u00a01\u00a02,\u00a0Joseph Grossman\u00a01\u00a02,\u00a0Raul Gonzalez\u00a07,\u00a0Sofia Perea Del Pino\u00a01\u00a02,\u00a0George Daaboul\u00a05,\u00a0Mandeep S Sawhney\u00a01\u00a02,\u00a0Steven D Freedman\u00a01\u00a02,\u00a0Alexander Kleger\u00a04,\u00a0Richard D Cummings\u00a06,\u00a0Andrew Emili\u00a03,\u00a0Lakshmi B Muthuswamy\u00a01\u00a02,\u00a0Manuel Hidalgo\u00a01\u00a02,\u00a0Senthil K Muthuswamy\u00a01\u00a02 [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":1413,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","_themeisle_gutenberg_block_has_review":false},"categories":[43],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.10 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>PDX-derived organoids model in vivo drug response and secrete biomarkers - Peaches Biotech<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/peaches.es\/en\/pdx-derived-organoids-model-in-vivo-drug-response-and-secrete-biomarkers\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PDX-derived organoids model in vivo drug response and secrete biomarkers - Peaches Biotech\" \/>\n<meta property=\"og:description\" content=\"Publicado por PubMed 2020 Nov 5;5(21):e135544. doi: 10.1172\/jci.insight.135544. 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